![]() And she kissed Shinkuro when she is 7 as the first time. She disputes Hozuki's first daughter Yuno over Shinkuro. She can usually behave crying with eye lotion, but she cannot lie the ones whom seriously treat her. She behaves childish suitably as her age, but she retaliates when she is carelessly treated or revolted. Her tendency seems to be implied by Tamaki. She is alienated from commonsenses by Okunoin's education and curious to ask Shinkuro her unknown things.īut her questions are often "too early to know" for seven-year-old girl therefore Shinkuro tends to be reluctant to answer her. She is sincere to her wrong behaviours or manners. Her personality is naive to herself, cheerful and indulgent, but she quite disapproves of solitude and thirsts to be fondled. She sincerely yearns Shinkuro as "interactive sweetheart" and indicates that she will marry him. She was taken to Shinkuro who Benika instinctively believesĪnd she is getting intimate to Shinkuro in the cohabitation. Murasaki answered, "I want to love something.". She is ignored in Kuhoin's family tree and didn't attend school.īut Benika Juzawa got in Okunoin for her friend Aoki's promise that "If a girl will be born, please realise her single wish." and asked Murasaki. Kuhoin's women are permanently locked up in Okunoin, the secluded retreat for only incubating children in order to mask the awful facts. It's caused by several times done and therefore, harmed their reproductions. She is officially known as the daughter of Renjo Kuhoin and his wife, but actually, her mother is Renjo's biological sister, Aoki.īecause Kuhoin can only gain biological children by incests. Read more about how to correctly acknowledge RSC content.Ryuuji Kuhouin (Brother)}} Murasaki Kuhouin is a beautiful, bright and indulgent seven-year-old girl who attends class 3 of the first year of public school as the 8th place.Ī daughter of Kuhoin, the most prominent clan of Omotegosanke. Please go to the Copyright Clearance Center request page. In a third-party publication (excluding your thesis/dissertation for which permission is not required) If you want to reproduce the whole article If you are the author of this article, you do not need to request permission to reproduce figuresĪnd diagrams provided correct acknowledgement is given. Provided correct acknowledgement is given. If you are an author contributing to an RSC publication, you do not need to request permission To request permission to reproduce material from this article, please go to the Synthesis and photophysical properties of a new push–pull pyrene dye with green-to-far-red emission and its application to human cellular and skin tissue imaging These results suggest that PC could replace several commonly used dyes in various biological applications, particularly the rapid and accurate diagnosis of tissue diseases, typified by biopsy. Interestingly, PC provides bright 2PM images under tissue-penetrative 960 nm excitation, realizing much clearer and deeper tissue imaging than conventional pyrene dyes and NR. Furthermore, PC efficiently stained normal human skin tissue blocks treated with a transparency-enhancing agent and enabled clear visualization of individual cells in each tissue architecture by means of two-photon fluorescence microscopy (2PM). ![]() Under the cell conditions where the formation of droplets was inhibited, PC could be distributed to both the remaining droplets and the intercellular membranes, which could be distinguished based on the fluorescence solvatochromic function of PC. When human prostate cancer cells stained with PC were imaged using a confocal laser scanning fluorescence microscope, PC was found to selectively stain the lipid droplets. The newly synthesized dye exhibits highly polarity-sensitive fluorescence over a significantly wide range ( i.e., the green to far-red region), accompanied by high fluorescence quantum yields ( Φ FL > 0.70 in most organic solvents) and superior photostability to that of the commonly used Nile Red ( NR) dye, which also fluoresces in the green to red region. Herein, we discuss a new pyrene-based push–pull dye ( PC) and our investigation of its photophysical properties and applicability to biological studies. ![]()
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